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recombinant human il 7  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec recombinant human il 7
    Recombinant Human Il 7, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/macs/MACS+GMP+Recombinant+Human+IL-7/pmc13651853-267-16-20
    Average 93 stars, based on 24 article reviews
    recombinant human il 7 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: Modulation of macrophage polarization with acute administration of Vericiguat after myocardial infarction.
    Article Snippet: 26 Vericiguat (Merck, marketed as Verquvo) is a soluble guanylate cyclase stimulator that is FDA-approved for 27 use in heart failure patients with reduced or mildly reduced ejection fraction (HFrEF, HFmrEF) to decrease 28 heart failure hospitalization and cardiovascular mortality.. We hypothesized that earlier administration of 29 vericiguat post-MI would reduce the workload of the viable cardiomyocytes, leading to an earlier switch 30 towards anti-inflammatory macrophages and reduced adverse remodeling.. Male and female C57BL/6J mice 31 (n=6/sex/group) underwent permanent occlusion of the left anterior descending coronary artery followed by 32 implantation of a subcutaneous osmotic mini-pump (vericiguat or saline) 24 hours later.

    Article Title: Viral protease-initiated lytic cell death as a universal antiviral mRNA therapy.
    Article Snippet: Article Viral protease-initiated lytic cell death as a universal antiviral mRNA therapy

    Article Title: TSC22D3-Mediated Quiescence Preservation Boosts HSC Engraftment in Xenografts
    Article Snippet: CD34+ HSPCs were isolated from mobilized peripheral blood of healthy volunteers using MACS (Miltenyi Biotec, Bergisch Gladbach, Germany, catalog No. 130-046-703), with approval from the Institutional Review Board (IRB) of Longhua District People’s Hospital of Shenzhen.

    Article Title: Naringenin ameliorates swine pulpitis by modulating immune response.
    Article Snippet: Human neutrophils (hNeu.) were isolated from peripheral blood using MACS (130- 104-434, Miltenyi Biotec) following the manufacturer’s instructions. hDPFs were cultured in α-minimal essential medium (MEM-α, Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Beyotime) at 37°C in a humidified atmosphere of 5% CO2.

    Article Title: Interferon-λ drives renal fibrosis by coordinating epithelial-fibroblast crosstalk.
    Article Snippet: Pellets were resuspended in MACS running buffer (130-091-221; Miltenyi Biotec) and incubated for 30 min with biotinylated primary anti-vimentin antibody (Biotin-60330; Proteintech).

    Article Title: High-density Lipoprotein Inspired Lipid Nanoparticles for Systemic RNA delivery to the Brain
    Article Snippet: Neural cell populations were enriched by MACS (Miltenyi Biotec) using the following kits according to the manufacturer’s instructions: Neuron Isolation Kit, mouse (130-115-389); CD11b (microglia) MicroBeads (130-093-634); Anti-ACSA-2 MicroBead Kit (130-097-678); Anti-O4 MicroBeads (130-094-543); and CD31 MicroBeads (130-097-418).

    Article Title: Ligand-Blocking and Agonist Antibodies Targeting TNFR2 Employ Distinct Modes of Action to Induce Antitumor Immunity
    Article Snippet: Mouse CD4+ T cells were isolated from 249 spleens by MACS (CD4+ T cell isola on kit (mouse), Miltenyi Cat# 130-104-454, RRID:AB_3716593).

    Magnetic Cell Separation:

    Article Title: Modulation of macrophage polarization with acute administration of Vericiguat after myocardial infarction.
    Article Snippet: 26 Vericiguat (Merck, marketed as Verquvo) is a soluble guanylate cyclase stimulator that is FDA-approved for 27 use in heart failure patients with reduced or mildly reduced ejection fraction (HFrEF, HFmrEF) to decrease 28 heart failure hospitalization and cardiovascular mortality.. We hypothesized that earlier administration of 29 vericiguat post-MI would reduce the workload of the viable cardiomyocytes, leading to an earlier switch 30 towards anti-inflammatory macrophages and reduced adverse remodeling.. Male and female C57BL/6J mice 31 (n=6/sex/group) underwent permanent occlusion of the left anterior descending coronary artery followed by 32 implantation of a subcutaneous osmotic mini-pump (vericiguat or saline) 24 hours later.

    Article Title: Viral protease-initiated lytic cell death as a universal antiviral mRNA therapy.
    Article Snippet: Article Viral protease-initiated lytic cell death as a universal antiviral mRNA therapy

    Article Title: TSC22D3-Mediated Quiescence Preservation Boosts HSC Engraftment in Xenografts
    Article Snippet: CD34+ HSPCs were isolated from mobilized peripheral blood of healthy volunteers using MACS (Miltenyi Biotec, Bergisch Gladbach, Germany, catalog No. 130-046-703), with approval from the Institutional Review Board (IRB) of Longhua District People’s Hospital of Shenzhen.

    Article Title: Naringenin ameliorates swine pulpitis by modulating immune response.
    Article Snippet: Human neutrophils (hNeu.) were isolated from peripheral blood using MACS (130- 104-434, Miltenyi Biotec) following the manufacturer’s instructions. hDPFs were cultured in α-minimal essential medium (MEM-α, Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Beyotime) at 37°C in a humidified atmosphere of 5% CO2.

    Article Title: Interferon-λ drives renal fibrosis by coordinating epithelial-fibroblast crosstalk.
    Article Snippet: Pellets were resuspended in MACS running buffer (130-091-221; Miltenyi Biotec) and incubated for 30 min with biotinylated primary anti-vimentin antibody (Biotin-60330; Proteintech).

    Article Title: High-density Lipoprotein Inspired Lipid Nanoparticles for Systemic RNA delivery to the Brain
    Article Snippet: Neural cell populations were enriched by MACS (Miltenyi Biotec) using the following kits according to the manufacturer’s instructions: Neuron Isolation Kit, mouse (130-115-389); CD11b (microglia) MicroBeads (130-093-634); Anti-ACSA-2 MicroBead Kit (130-097-678); Anti-O4 MicroBeads (130-094-543); and CD31 MicroBeads (130-097-418).

    Article Title: Ligand-Blocking and Agonist Antibodies Targeting TNFR2 Employ Distinct Modes of Action to Induce Antitumor Immunity
    Article Snippet: Mouse CD4+ T cells were isolated from 249 spleens by MACS (CD4+ T cell isola on kit (mouse), Miltenyi Cat# 130-104-454, RRID:AB_3716593).

    Cell Culture:

    Article Title: Modulation of macrophage polarization with acute administration of Vericiguat after myocardial infarction.
    Article Snippet: 26 Vericiguat (Merck, marketed as Verquvo) is a soluble guanylate cyclase stimulator that is FDA-approved for 27 use in heart failure patients with reduced or mildly reduced ejection fraction (HFrEF, HFmrEF) to decrease 28 heart failure hospitalization and cardiovascular mortality.. We hypothesized that earlier administration of 29 vericiguat post-MI would reduce the workload of the viable cardiomyocytes, leading to an earlier switch 30 towards anti-inflammatory macrophages and reduced adverse remodeling.. Male and female C57BL/6J mice 31 (n=6/sex/group) underwent permanent occlusion of the left anterior descending coronary artery followed by 32 implantation of a subcutaneous osmotic mini-pump (vericiguat or saline) 24 hours later.

    Article Title: Viral protease-initiated lytic cell death as a universal antiviral mRNA therapy.
    Article Snippet: Article Viral protease-initiated lytic cell death as a universal antiviral mRNA therapy

    Article Title: TSC22D3-Mediated Quiescence Preservation Boosts HSC Engraftment in Xenografts
    Article Snippet: CD34+ HSPCs were isolated from mobilized peripheral blood of healthy volunteers using MACS (Miltenyi Biotec, Bergisch Gladbach, Germany, catalog No. 130-046-703), with approval from the Institutional Review Board (IRB) of Longhua District People’s Hospital of Shenzhen.

    Article Title: Naringenin ameliorates swine pulpitis by modulating immune response.
    Article Snippet: Human neutrophils (hNeu.) were isolated from peripheral blood using MACS (130- 104-434, Miltenyi Biotec) following the manufacturer’s instructions. hDPFs were cultured in α-minimal essential medium (MEM-α, Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Beyotime) at 37°C in a humidified atmosphere of 5% CO2.

    Article Title: Interferon-λ drives renal fibrosis by coordinating epithelial-fibroblast crosstalk.
    Article Snippet: Pellets were resuspended in MACS running buffer (130-091-221; Miltenyi Biotec) and incubated for 30 min with biotinylated primary anti-vimentin antibody (Biotin-60330; Proteintech).

    Article Title: High-density Lipoprotein Inspired Lipid Nanoparticles for Systemic RNA delivery to the Brain
    Article Snippet: Neural cell populations were enriched by MACS (Miltenyi Biotec) using the following kits according to the manufacturer’s instructions: Neuron Isolation Kit, mouse (130-115-389); CD11b (microglia) MicroBeads (130-093-634); Anti-ACSA-2 MicroBead Kit (130-097-678); Anti-O4 MicroBeads (130-094-543); and CD31 MicroBeads (130-097-418).

    Article Title: Ligand-Blocking and Agonist Antibodies Targeting TNFR2 Employ Distinct Modes of Action to Induce Antitumor Immunity
    Article Snippet: Mouse CD4+ T cells were isolated from 249 spleens by MACS (CD4+ T cell isola on kit (mouse), Miltenyi Cat# 130-104-454, RRID:AB_3716593).



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    Astrocytic sirtuin2 (Sirt2) deficiency attenuates inflammatory astrocyte programs and enhances repair-associated features. (A) Single-cell RNA sequencing (scRNA-seq) workflow for injured spinal cords from control and Sirt2 conditional knockout (cKO) mice at 14 d post-injury (dpi). (B) Uniform manifold approximation and projection (UMAP) of major cell populations. (C and D) Astrocyte reclustering into groups enriched for resting-associated, A1-associated, and A2-associated marker programs. (E and F) Relative proportions of the transcriptionally defined astrocyte groups in control and Sirt2 cKO samples. (G and H) Bulk RNA sequencing (RNA-seq) of MACS-purified astrocytes showing A1-associated and A2-associated markers and representative differentially expressed genes. (I) Gene Ontology (GO) analysis of genes up-regulated in magnetic-activated cell sorting (MACS)-purified Sirt2 cKO astrocytes by bulk RNA-seq. (J and K) Ki67 (purple), glial fibrillary acidic protein (GFAP; green), and 4′,6-diamidino-2-phenylindole (DAPI; blue) staining and quantification at 14 dpi. Scale bar, 20 μm. (L and M) C3 (purple), GFAP (green), and DAPI (blue) staining and quantification. Scale bars, 100 μm for overview images and 20 μm for insets. (N and O) S100A10 (purple), GFAP (green), and DAPI (blue) staining and quantification. Scale bars, 100 μm for overview images and 20 μm for insets. Data are mean ± standard error of the mean (SEM); n = 5 for (K), (M), and (O). For scRNA-seq, 8 mice per group were pooled. A 2-tailed unpaired Student t test was used for (K), (M), and (O). **** P < 0.0001.

    Journal: Research

    Article Title: Astrocytic Sirt2-Mediated Delactylation Limits Blood–Spinal Cord Barrier Reconstruction after Spinal Cord Injury

    doi: 10.34133/research.1447

    Figure Lengend Snippet: Astrocytic sirtuin2 (Sirt2) deficiency attenuates inflammatory astrocyte programs and enhances repair-associated features. (A) Single-cell RNA sequencing (scRNA-seq) workflow for injured spinal cords from control and Sirt2 conditional knockout (cKO) mice at 14 d post-injury (dpi). (B) Uniform manifold approximation and projection (UMAP) of major cell populations. (C and D) Astrocyte reclustering into groups enriched for resting-associated, A1-associated, and A2-associated marker programs. (E and F) Relative proportions of the transcriptionally defined astrocyte groups in control and Sirt2 cKO samples. (G and H) Bulk RNA sequencing (RNA-seq) of MACS-purified astrocytes showing A1-associated and A2-associated markers and representative differentially expressed genes. (I) Gene Ontology (GO) analysis of genes up-regulated in magnetic-activated cell sorting (MACS)-purified Sirt2 cKO astrocytes by bulk RNA-seq. (J and K) Ki67 (purple), glial fibrillary acidic protein (GFAP; green), and 4′,6-diamidino-2-phenylindole (DAPI; blue) staining and quantification at 14 dpi. Scale bar, 20 μm. (L and M) C3 (purple), GFAP (green), and DAPI (blue) staining and quantification. Scale bars, 100 μm for overview images and 20 μm for insets. (N and O) S100A10 (purple), GFAP (green), and DAPI (blue) staining and quantification. Scale bars, 100 μm for overview images and 20 μm for insets. Data are mean ± standard error of the mean (SEM); n = 5 for (K), (M), and (O). For scRNA-seq, 8 mice per group were pooled. A 2-tailed unpaired Student t test was used for (K), (M), and (O). **** P < 0.0001.

    Article Snippet: Tissues were kept in MACS Tissue Storage Solution (Miltenyi Biotec), rinsed with PBS, minced on ice into approximately 1-mm 3 fragments, and digested for 30 min at 37 °C with agitation in papain (20 U/ml) and DNase I (30 U/ml).

    Techniques: Single Cell, RNA Sequencing, Control, Gene Knockout, Marker, Bulk RNA Sequencing, Purification, Analysis, FACS, Staining

    (A) Schematic representation of the ex vivo SC assays ( ev SCAs). Parasite isolates from RBCs with different HBB genotypes cultured ex vivo , ML10 added at 11 hpi., MACS-enriched stage I gametocytes and schizonts detected by IFA Pfs16 (sexual stages) and DAPI (total parasites) staining. SCR (%): number of sexual stages/number of total parasites. (B) Representative IFA images of parasites in HbAA, HbAC and HbAS RBCs. Top row with DAPI (all parasites; blue arrows: schizonts) and AF488 (white arrows: stage I gametocytes) overlay and bottom row including brightfield (BF) channel to visualize the parasite’s hemozoin. Objective: 40 × ; Scale bar: 5µm. (C) SCRs by HBB genotype determined using IFA. Individual dots, represent the mean SCR of three independent technical IFA replicates from one culture; the horizontal line and error bars indicate the median SCR and IQR of all cultures from the same HBB genotype. (D) Initial parasitemia at 0 hpc by HBB genotype Statistical tests: C, Beta-binomial GL(M)M with per-genotype dispersion parameter and Bonferroni correction for pairwise comparisons; D, Kruskal-Wallis test. Significant ( p < 0.05*, p < 0.01**, p < 0.001***) and non-significant p-values (ns). Abbreviations: AF, Alexa Fluor; DAPI, 4’,6-diamidino-2-phenylindole; ev SCAs, ex vivo sexual conversion assays; GL(M)Ms, Generalized linear (mixed) models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpc, hours post culture; hpi, hours post-invasion; IFA, immunofluorescence assays; MACS, magnetic activated cell separation; ML10, cGMP-dependent protein kinase inhibitor; RBCs, red blood cells; SC, sexual conversion; SCR, sexual conversion rate. Panel A was created in BioRender. Yasmina, D. (2026) https://BioRender.com/f0i2udf .

    Journal: PLOS Pathogens

    Article Title: Elevated Plasmodium falciparum sexual conversion in HbAC and HbAS red blood cells in naturally infected malaria patients

    doi: 10.1371/journal.ppat.1014542

    Figure Lengend Snippet: (A) Schematic representation of the ex vivo SC assays ( ev SCAs). Parasite isolates from RBCs with different HBB genotypes cultured ex vivo , ML10 added at 11 hpi., MACS-enriched stage I gametocytes and schizonts detected by IFA Pfs16 (sexual stages) and DAPI (total parasites) staining. SCR (%): number of sexual stages/number of total parasites. (B) Representative IFA images of parasites in HbAA, HbAC and HbAS RBCs. Top row with DAPI (all parasites; blue arrows: schizonts) and AF488 (white arrows: stage I gametocytes) overlay and bottom row including brightfield (BF) channel to visualize the parasite’s hemozoin. Objective: 40 × ; Scale bar: 5µm. (C) SCRs by HBB genotype determined using IFA. Individual dots, represent the mean SCR of three independent technical IFA replicates from one culture; the horizontal line and error bars indicate the median SCR and IQR of all cultures from the same HBB genotype. (D) Initial parasitemia at 0 hpc by HBB genotype Statistical tests: C, Beta-binomial GL(M)M with per-genotype dispersion parameter and Bonferroni correction for pairwise comparisons; D, Kruskal-Wallis test. Significant ( p < 0.05*, p < 0.01**, p < 0.001***) and non-significant p-values (ns). Abbreviations: AF, Alexa Fluor; DAPI, 4’,6-diamidino-2-phenylindole; ev SCAs, ex vivo sexual conversion assays; GL(M)Ms, Generalized linear (mixed) models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpc, hours post culture; hpi, hours post-invasion; IFA, immunofluorescence assays; MACS, magnetic activated cell separation; ML10, cGMP-dependent protein kinase inhibitor; RBCs, red blood cells; SC, sexual conversion; SCR, sexual conversion rate. Panel A was created in BioRender. Yasmina, D. (2026) https://BioRender.com/f0i2udf .

    Article Snippet: At ~24 hpc, parasites were harvested and purified using MACS LD columns (Miltenyi Biotec).

    Techniques: Ex Vivo, Parasite, Cell Culture, Staining, Brightfield, Dispersion, Immunofluorescence